Translational_Unit

Part:BBa_K4912012

Designed by: Jiang Yunchong   Group: iGEM23_HUBU-HUBEI-CHINA   (2023-10-10)


Key enzymes for synthesizing hydroxytyrosol

To optimize the expression intensity of kivD and hpaBC in the promoter, the promoter was changed, as detailed in the parts BBa_K4912009 and BBa_K4912008


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1093
    Illegal PstI site found at 2354
    Illegal PstI site found at 3695
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1093
    Illegal PstI site found at 2354
    Illegal PstI site found at 3695
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1093
    Illegal BglII site found at 869
    Illegal BglII site found at 2045
    Illegal BglII site found at 2370
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1093
    Illegal PstI site found at 2354
    Illegal PstI site found at 3695
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1093
    Illegal PstI site found at 2354
    Illegal PstI site found at 3695
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4150


[edit]
Categories
//chassis/prokaryote
Parameters
biology